Difference between revisions of "Part:BBa K896999:Design"
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===References=== | ===References=== | ||
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| + | [Zheng09] Zheng ''et al'' (2009) Purification and Functional Characterization of ΦX174 Lysis Protein E, Biochemistry, 48 (22), pp 4999–5006 | ||
Latest revision as of 04:20, 5 April 2012
ΦX174; E (lysis) gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers used in the PCR:
X174_E_FP: gcttctag atggtacgctggactttgt (TM:55C) X174_E_RP: gct ctgcagcggccgctactagta tcactccttccgcacg (TM:55C)
Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.
Source
PCR'd from ΦX174 wildtype phage.
References
[Zheng09] Zheng et al (2009) Purification and Functional Characterization of ΦX174 Lysis Protein E, Biochemistry, 48 (22), pp 4999–5006