Difference between revisions of "Part:BBa J176131:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_J176131 short</partinfo>
 
<partinfo>BBa_J176131 short</partinfo>
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===Design Notes===
 
===Design Notes===
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TBA
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* The peptide sequence is repetitive [EAAAR][EAAAR][EAAAR][EAAAR] (Yan et al., 2007). In order to reduce repetition at the DNA sequence level, human-optimazed codons were distributed randomly along the sequence. This was done using a permutation tool I found online, along with some tedious manual text editing to create more variation where the sequence still looked too repetitive
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* De novo DNA synthesis was provided by Genscript
  
  
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===References===
 
===References===
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# Yan W, Imanishi M, Futaki S, Sugiura Y. (2007) alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence. Biochem. 46: 8517-8524

Latest revision as of 21:58, 7 March 2012

PLrigid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The peptide sequence is repetitive [EAAAR][EAAAR][EAAAR][EAAAR] (Yan et al., 2007). In order to reduce repetition at the DNA sequence level, human-optimazed codons were distributed randomly along the sequence. This was done using a permutation tool I found online, along with some tedious manual text editing to create more variation where the sequence still looked too repetitive
  • De novo DNA synthesis was provided by Genscript


Source

TBA

References

  1. Yan W, Imanishi M, Futaki S, Sugiura Y. (2007) alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence. Biochem. 46: 8517-8524