Difference between revisions of "Part:BBa J176131"

Revision as of 21:48, 7 March 2012

PLrigid

(EAAAR)4 peptide linker, rigid; alpha helix secondary structure

Usage and Biology

PLrigid is a short peptide that has an alpha-helix structure. We use it as a "linker" to connect protein domains/ modules. Alpha helices are thought to be rigid structures that restrict the position of one end relative to the other. Lack of "bendability" keeps the two proteins attached at either end from coming closer together. Since the proteins are also unable to move farther apart when they are attached with a rigid linker, the relative position of the two proteins is fixed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 20
Illegal PstI site found at 35
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 20
Illegal PstI site found at 35
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 20
Illegal PstI site found at 35
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 20
Illegal PstI site found at 35
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J176131 short</partinfo>
-(EAAAR)4 peptide linker, rigid; alpha helix secondary structure; see Yan et al. 2007
+(EAAAR)4 peptide linker, rigid; alpha helix secondary structure
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+PLrigid is a short peptide that has an alpha-helix structure. We use it as a "linker" to connect protein domains/ modules. Alpha helices are thought to be rigid structures that restrict the position of one end relative to the other. Lack of "bendability" keeps the two proteins attached at either end from coming closer together. Since the proteins are also unable to move farther apart when they are attached with a rigid linker, the relative position of the two proteins is fixed.
 <!-- -->