Difference between revisions of "Part:BBa J176017"

Revision as of 21:34, 18 February 2012

CMVTetO2

Doxycycline-inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_J176017 short</partinfo>
-complete tet./ dox. inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system
+Doxycycline-inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+* Chassis: mammalian cells
+* When used in cells without Tet, the promoter is constitutive (always on)
+* Tetracycline is also an effective inducer. The levels of tetracycline in tet-treated cows is sufficient to cause a leaky off-state. Tet-free fetal bovine serum is highly recommended.
+<br>
+'''CMVTetO2''' is a hybrid DNA element composed of the naturally occurring cytomegalovirus promoter (See CMV, <partinfo>BBa_J176027</partinfo>) and two tetracycline repressor binding sites/ operators (TetO x2). The TATA box motif stimulates formation of the transcription initiation complex at the promoter. The off-state of the promoter is maintained when the Tet repressor protein (expressed from a different gene) binds to the TetO x2 sites. When doxycycline or tetracycline is added to the cell culture medium, the chemical binds the Tet repressor, induces an allosteric change that decreases the repressor's affinity for the binding sites, and the promoter become active.
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