Difference between revisions of "Part:BBa J153003"

 
 
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<partinfo>BBa_J153003 short</partinfo>
 
<partinfo>BBa_J153003 short</partinfo>
  
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Uses the strong Ptrc1O promoter (link) to generate untagged GFPmut3b (link).
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Uses the strong Ptrc1O promoter ([[Part:BBa_J153001]]) to generate untagged GFPmut3b ([[Part:BBa_E0040]]).
  
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Usage
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===Usage and Biology===
  
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This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (link) in various cyanobacteria (Huang etal 2010) (link). This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) (link).
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This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 ([[Part:BBa_J153000]]) in various cyanobacteria (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988]. This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) [http://www.ncbi.nlm.nih.gov/pubmed/21204939].
  
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===Usage and Biology===
 
  
 
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Latest revision as of 15:03, 3 February 2012

Ptrc1O-driven GFP reporter

Uses the strong Ptrc1O promoter (Part:BBa_J153001) to generate untagged GFPmut3b (Part:BBa_E0040).

Usage and Biology

This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) in various cyanobacteria (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988]. This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) [http://www.ncbi.nlm.nih.gov/pubmed/21204939].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733