Difference between revisions of "Part:BBa J153002"

Revision as of 12:12, 3 February 2012

Ptrc2O

Ptrc2O is a modified version of Ptrc1O (Part:BBa_J153001) that contains two lac operators to allow for stronger repression through DNA looping by the LacI tetramer.

It contains the lac O1 operator (proximal to the core promoter) and the ideal/symmetrical lac operator (distal to the core promoter) that allow for LacI repression and subsequent induction by IPTG.

Usage and Biology

It was characterized with a GFP reporter construct (Part:BBa_I13504) carried on the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) to be of similar strength and repressibility as Ptrc1O in E. coli DH5alpha. Characterization in the cyanobacterium Synechocystis sp. PCC 6803 revealed strong fluorescence from the Ptrc2O-driven construct, similar to the Ptrc1O-driven construct. However, as compared to Ptrc1O, it was strongly repressed by LacI, but could on the other hand not be induced by IPTG to its full strength (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J153002 short</partinfo>
-Ptrc2O is a modified version of Ptrc1O (link) that contains two lac operators to allow for stronger repression through DNA looping.
+P''trc2O'' is a modified version of P''trc1O'' ([[Part:BBa_J153001]]) that contains two ''lac'' operators to allow for stronger repression through DNA looping by the LacI tetramer.
-It contains the lac O1 operator (proximal to the core promoter) and the ideal/symmetrical lac operator (distal to the core promoter) that allow for LacI repression and subsequent induction by IPTG.
-Usage
-It was characterized with a GFP reporter construct (link) carried on the broad-host-range shuttle vector pPMQAK1 (link) to be of similar strength and repressibility as Ptrc1O in E. coli DH5alpha. Characterization in the cyanobacterium Synechocystis sp. PCC 6803 revealed strong fluorescence from the Ptrc2O-driven construct, similar to the Ptrc1O-driven construct. However, as compared to Ptrc1O, it was strongly repressed by LacI, but could on the other hand not be induced by IPTG to its full strength (Huang etal 2010) (link).
+It contains the ''lac'' O1 operator (proximal to the core promoter) and the ideal/symmetrical ''lac'' operator (distal to the core promoter) that allow for LacI repression and subsequent induction by IPTG.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+It was characterized with a GFP reporter construct ([[Part:BBa_I13504]]) carried on the broad-host-range shuttle vector pPMQAK1 ([[Part:BBa_J153000]]) to be of similar strength and repressibility as P''trc1O'' in ''E. coli'' DH5alpha. Characterization in the cyanobacterium ''Synechocystis'' sp. PCC 6803 revealed strong fluorescence from the P''trc2O''-driven construct, similar to the P''trc1O''-driven construct. However, as compared to P''trc1O'', it was strongly repressed by LacI, but could on the other hand not be induced by IPTG to its full strength (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988].
 <!-- -->