Difference between revisions of "Part:BBa J176137"

 
 
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Red fluorescent protein designed to be repressed by microRNAs (miR's) from luciferase (artificial) and miR-34 (natural)<br>
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Alias: DF5
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===Usage and Biology===
 
===Usage and Biology===
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Double mCherry red fluorescent protein that enters the nucleus (via the NLS tag) and binds to Gal4 DNA sequences (via Gal4DB). It is designed to be repressed by either miR-34 (via miRts CCND1) or miR-luc (via miRts luc).
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Latest revision as of 04:13, 11 December 2011

HPK-RFP-miRts CCND1

Red fluorescent protein designed to be repressed by microRNAs (miR's) from luciferase (artificial) and miR-34 (natural)
Alias: DF5


Usage and Biology

Double mCherry red fluorescent protein that enters the nucleus (via the NLS tag) and binds to Gal4 DNA sequences (via Gal4DB). It is designed to be repressed by either miR-34 (via miRts CCND1) or miR-luc (via miRts luc).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1116
    Illegal NgoMIV site found at 3249
    Illegal AgeI site found at 116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 755