Difference between revisions of "Part:BBa M36295:Design"

 
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__NOTOC__
 
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<partinfo>BBa_M36295 short</partinfo>
 
<partinfo>BBa_M36295 short</partinfo>
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===Source===
 
===Source===
  
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RBS Leader and ATG came from BioFAB. Terminator comes from BioFAB. FOS-JUN leucine zipper sequences were taken from O'Shea et al. They were also codon optimized for E.coli.
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Escherichia coli
  
 
===References===
 
===References===

Latest revision as of 00:55, 11 December 2011

Spider Silk GFP Actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 898


Design Notes

The overall design had a 3000 base pair limit. FOS and JUN leucine zippers were used to covalently bond GFP proteins to create a larger strand.


Source

Escherichia coli

References