Difference between revisions of "Part:BBa M36295"

 
 
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<partinfo>BBa_M36295 short</partinfo>
 
<partinfo>BBa_M36295 short</partinfo>
  
This is the full sequence for the spider silk GFP actuator. It was the positive control to test if the FOS and JUN sequences worked. It includes the bicistonic sequence, FOS, a linker, GFP, another linker, JUN, a stop codon, and a transcription terminator. It is triggered by PoPS.
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The input for this composite is the PoPs signal generated by rhamnose, and the output is GFP aggregates. This produces GFP molecules linked in long chains. Fos and Jun are on either side of the GFP DNA sequence and link together to create the long chains. This composite specifically tests whether Fos and Jun linkages were working to heterodimerize the GFP monomers.
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The RBS bicistronic sequence is of medium strength. The linkers are very short so the mRNA does not fold on itself if there are complimentary parts. The transcription terminator has 99% efficiency because we wanted to ensure transcription would stop.
  
 
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Latest revision as of 00:55, 11 December 2011

Spider Silk GFP Actuator

The input for this composite is the PoPs signal generated by rhamnose, and the output is GFP aggregates. This produces GFP molecules linked in long chains. Fos and Jun are on either side of the GFP DNA sequence and link together to create the long chains. This composite specifically tests whether Fos and Jun linkages were working to heterodimerize the GFP monomers.

The RBS bicistronic sequence is of medium strength. The linkers are very short so the mRNA does not fold on itself if there are complimentary parts. The transcription terminator has 99% efficiency because we wanted to ensure transcription would stop.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 898