Difference between revisions of "Part:BBa M36304"

 
m
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_M36304 short</partinfo>
 
<partinfo>BBa_M36304 short</partinfo>
  
−
The part is (enter text here).
+
Constitutive promoter bounded by four FseI sites. The expected operational result is that the promoter will be spliced out of a plasmid when FseI is present.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
Place this part next to a gene of interest so that the gene is constitutively expressed until the restriction endonuclease FseI is present. Effectively, this part will work to knock out a gene.
  
 
<!-- -->
 
<!-- -->

Latest revision as of 07:22, 10 December 2011

Constitutive promoter with FseI restriction sites

Constitutive promoter bounded by four FseI sites. The expected operational result is that the promoter will be spliced out of a plasmid when FseI is present.

Usage and Biology

Place this part next to a gene of interest so that the gene is constitutively expressed until the restriction endonuclease FseI is present. Effectively, this part will work to knock out a gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 93
    Illegal NgoMIV site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]