Difference between revisions of "Part:BBa M36307"

 
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<partinfo>BBa_M36307 short</partinfo>
 
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This composite part includes a strong bicistronic RBS, the FseI protein coding gene, and the strong pT-T7 terminator.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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The coding region produces the restriction enzyme FseI. As FseI is produced, it cleaves DNA at recognition sites using exonuclease activity. This can be utilized for experiments by surrounding a gene of interest with FseI cut sites. Under the control of a promoter of choice, FseI can be expressed and cut out either the promoter or entire gene in the gene of interest. This way, one can knock out genes at specific times.
  
 
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Revision as of 07:07, 10 December 2011

FseI restriction endonuclease actuator construct

This composite part includes a strong bicistronic RBS, the FseI protein coding gene, and the strong pT-T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 595
    Illegal NheI site found at 761
    Illegal PstI site found at 532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 431
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 532
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 94