Difference between revisions of "Part:BBa M36394"

Latest revision as of 23:56, 8 December 2011

Spider Silk Actuator

Our part takes as input the PoPS signal generated by rhamnose, and the output is spider silk protein. This is a spider silk generating device that produces a hexamer of spider silk protein codon optimized for expression in E. coli. The DNA of the device is made up of Fos and Jun linked to the front and back of the spider silk hexamer "spilk 7.0." Fos and Jun are proteins with leucine zippers that form heterodimers in solution. The idea is that our E. coli cell will produce the hexamers of spider silk and have them aggregate together in very long chains bound together by Fos and Jun interactions.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 109
Illegal PstI site found at 151
Illegal PstI site found at 510
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 109
Illegal PstI site found at 151
Illegal PstI site found at 510
Illegal NotI site found at 302
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 109
Illegal PstI site found at 151
Illegal PstI site found at 510
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 109
Illegal PstI site found at 151
Illegal PstI site found at 510
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 663

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 <partinfo>BBa_M36394 short</partinfo>
-This is a spider silk generating device that produces a hexamer of spider silk protein codon optimized for expression in E. coli. The DNA of the device is made up of Fos  and Jun linked to the front and back of the spider silk hexamer "spilk 7.0.2." Fos and Jun are proteins with leucine zippers that form heterodimers in solution. The idea is that our E. coli cell will produce the hexamers of spider silk and have them aggregate together in very long chains bound together by Fos and Jun interactions. Our part was placed in a Rhamnex 67K Xbrane plasmid, meaning that the device is activated by the PoPS generated by the rhaBAD promoter system.
+Our part takes as input the PoPS signal generated by rhamnose, and the  output is spider silk protein. This is a spider silk generating device that produces a hexamer of spider silk protein codon optimized for expression in E. coli. The DNA of the device is made up of Fos  and Jun linked to the front and back of the spider silk hexamer "spilk 7.0." Fos and Jun are proteins with leucine zippers that form heterodimers in solution. The idea is that our E. coli cell will produce the hexamers of spider silk and have them aggregate together in very long chains bound together by Fos and Jun interactions.
 <!-- Add more about the biology of this part here