Difference between revisions of "Part:BBa J176022:Design"

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__NOTOC__
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<partinfo>BBa_J176022 short</partinfo>
  
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<partinfo>BBa_J176022 SequenceAndFeatures</partinfo>
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===Design Notes===
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* PCR-cloned from pAmCyan1-C1 (Clontech)
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* Primers add a XbaI site upstream and SpeI/NotI/PstI downstream:
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** Forward 5'-CCTTTCTAGAatggccctgtccaac
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** Reverse 5'-AAGGCTGCAGCGGCCGCTACTAGTgaagggcaccacgga
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* Mutated at position 510 to remove a PstI site (site directed mutagenesis primer 5'-gatgctgcaAggcggcggca)
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===Source===
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* pAmCyan1-C1 (Clontech)
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===References===
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* Matz, M. V., et al. (1999) Nature Biotech. 17:969–973

Latest revision as of 23:25, 8 December 2011

AmCyan


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR-cloned from pAmCyan1-C1 (Clontech)
  • Primers add a XbaI site upstream and SpeI/NotI/PstI downstream:
    • Forward 5'-CCTTTCTAGAatggccctgtccaac
    • Reverse 5'-AAGGCTGCAGCGGCCGCTACTAGTgaagggcaccacgga
  • Mutated at position 510 to remove a PstI site (site directed mutagenesis primer 5'-gatgctgcaAggcggcggca)


Source

  • pAmCyan1-C1 (Clontech)


References

  • Matz, M. V., et al. (1999) Nature Biotech. 17:969–973