Difference between revisions of "Part:BBa M36549"

(Usage and Biology)
 
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This composite part includes a medium-weak RBS, N4-derived gene (gp8), and a strong transcriptional terminator.  
 
This composite part includes a medium-weak RBS, N4-derived gene (gp8), and a strong transcriptional terminator.  
  
https://static.igem.org/mediawiki/igem.org/d/d5/M36549.png
+
https://static.igem.org/mediawiki/parts/d/d5/M36549.png
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 23:07, 8 December 2011

PoPS --> N4 gp8 (DNA replication inhibitor)

This composite part includes a medium-weak RBS, N4-derived gene (gp8), and a strong transcriptional terminator.

M36549.png

Usage and Biology

The coding region produces a small protein (gp8) which binds to the delta-subunit of DNA Polymerase III in the K12 strain of E. Coli to stop DNA replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]