Difference between revisions of "Part:BBa M36549:Design"
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===References=== | ===References=== | ||
Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338. | Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338. | ||
| + | Giacalone, M.J., Gentile, A.M., Lovitt, B.T., Berkley, N.L., Gunderson, C.W. and M.W. Surber. (2006), Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. ''BioTechniques'', 40: 355-364. | ||
Revision as of 22:32, 8 December 2011
PoPS --> N4 gp8 (DNA replication inhibitor)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part was tested in K12 E.coli on a vector with ampicillin resistance and a rhamnose-inducible and glucose-repressible sensor. This sensor contained rhaR, rhaS, and promoter PRHA (see reference 2 below for more imformation), and was physically directly before the RBS of the construct.
Source
[http://www.parts.igem.org Registry of Standard Biological Parts]
[http://biofab.jbei.org/services/studio/dac/ BIOFAB Data Access Client]
References
Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338. Giacalone, M.J., Gentile, A.M., Lovitt, B.T., Berkley, N.L., Gunderson, C.W. and M.W. Surber. (2006), Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. BioTechniques, 40: 355-364.