Difference between revisions of "Part:BBa M36548:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We derived this sequence from coliphage N4 gp8. | + | We derived this sequence from coliphage N4 gp8. Since N4 infects ''E. coli'', no codon optimization for ''E. coli'' is required. |
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| + | Since this function inhibits DNA replication, we advise using a medium- to low- strength RBS to prevent leaky expression during construction process. | ||
===Source=== | ===Source=== | ||
Latest revision as of 21:25, 8 December 2011
DNA Polymerase Inhibitor (via N4 coliphage gp8)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We derived this sequence from coliphage N4 gp8. Since N4 infects E. coli, no codon optimization for E. coli is required.
Since this function inhibits DNA replication, we advise using a medium- to low- strength RBS to prevent leaky expression during construction process.
Source
Coliphage N4 gp8
References
Yano, S.T. and L.B. Rothman-Denes (2011), A phage-encoded inhibitor of Escherichia coli DNA replication targets the DNA polymerase clamp loader. Molecular Microbiology, 79: 1325-1338.