Difference between revisions of "Part:BBa M36394:Experience"
(→Applications of BBa_M36394) |
(→Applications of BBa_M36394) |
||
| Line 4: | Line 4: | ||
===Applications of BBa_M36394=== | ===Applications of BBa_M36394=== | ||
| − | Alhough we were unable to isolate spider silk protein produced by the E. coli cells, we did conduct dosage response assays on our system. We made use of Congo Red, a dye that binds to amyloid like proteins such as spider silk, to test for the presence of the protein in cell lysate at 541 nm. Altough we went off the methodology of Slotta et al. for usage of Congo Red, we were unable to isolate the spider silk protein in the limited time for the project. Thus, we conducted a primary assay | + | Alhough we were unable to isolate spider silk protein produced by the E. coli cells, we did conduct dosage response assays on our system. We made use of Congo Red, a dye that binds to amyloid like proteins such as spider silk, to test for the presence of the protein in cell lysate at 541 nm. Altough we went off the methodology of Slotta et al. for usage of Congo Red, we were unable to isolate the spider silk protein in the limited time for the project. Thus, we conducted a primary assay to determine the ideal volume of cell lysate volume to add to Congo Red buffer before conducting a secondary assay adding this ideal volume of cell lysate to varying concentrations of rhamnose. |
| + | |||
| + | [[Image:primary assay.jpg]] | ||
| + | [[Image:secondary assay.jpg]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 03:23, 7 December 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M36394
Alhough we were unable to isolate spider silk protein produced by the E. coli cells, we did conduct dosage response assays on our system. We made use of Congo Red, a dye that binds to amyloid like proteins such as spider silk, to test for the presence of the protein in cell lysate at 541 nm. Altough we went off the methodology of Slotta et al. for usage of Congo Red, we were unable to isolate the spider silk protein in the limited time for the project. Thus, we conducted a primary assay to determine the ideal volume of cell lysate volume to add to Congo Red buffer before conducting a secondary assay adding this ideal volume of cell lysate to varying concentrations of rhamnose.
User Reviews
UNIQ8b8538d15f2265ac-partinfo-00000000-QINU UNIQ8b8538d15f2265ac-partinfo-00000001-QINU