Difference between revisions of "Part:BBa M36280:Design"

(→‎Design Notes)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
P00325 (ADH1B_HUMAN) provided by UniProt.
+
Homo Sapiens
  
 
===References===
 
===References===

Revision as of 23:12, 6 December 2011

Human Alcohol Dehydrogenase 1B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 367
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 367
    Illegal NgoMIV site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been codon optimized for high expression in E. Coli. We added the histidine tag after the alcohol dehydrogenase sequence to allow for enzyme purification by nickel affinity chromatography.

Source

Homo Sapiens

References