Difference between revisions of "Part:BBa M36280:Design"
(→Design Notes) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M36280 short</partinfo> | <partinfo>BBa_M36280 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This sequence has been codon optimized for high expression in E. Coli. | + | This sequence has been codon optimized for high expression in E. Coli. We added the histidine tag after the alcohol dehydrogenase sequence to allow for enzyme purification by nickel affinity chromatography. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 22:48, 6 December 2011
Human Alcohol Dehydrogenase 1B
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 367
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 367
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 367
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 367
Illegal NgoMIV site found at 885 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence has been codon optimized for high expression in E. Coli. We added the histidine tag after the alcohol dehydrogenase sequence to allow for enzyme purification by nickel affinity chromatography.
Source
P00325 (ADH1B_HUMAN) provided by UniProt.