Difference between revisions of "Part:BBa J176013"

(→‎Usage and Biology)
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
  
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[[Image:KAH_VP64.png|thumb|left|350px|'''The VP64 Activation Domain Module''' is often fused to DNA binding domains to make strong synthetic transcription factors. Figure adapted from Beerli et al., 1998.]]
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[[Image:KAH_VP64.png|thumb|left|350px|'''The VP64 Activation Domain Module''']]
  
 
* Chassis: mammalian cells
 
* Chassis: mammalian cells

Revision as of 17:51, 22 November 2011

VP64

Tetrameric VP16 transcription activator domain

Usage and Biology

The VP64 Activation Domain Module
  • Chassis: mammalian cells
  • Mammalian expression vector required
  • Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
  • VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain for the best results


VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447, DALDDFDLDML) connected with glycine-serine linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool, which pre-dates fancy terms like "synthetic biology." See the References section for some history on VP16 and VP64.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]