Difference between revisions of "Part:BBa K631031"

Revision as of 23:20, 9 October 2011 (view source) AllisterSmith (Talk | contribs) ← Older edit		Latest revision as of 13:33, 6 November 2011 (view source) AllisterSmith (Talk | contribs)
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	<b>Result</b><br>		<b>Result</b><br>
−	Characterization results have not yet been completed, however preliminary results involving the chemical 4-chlorobenzoic acid appear very promising. Comprehensive testing and characterization involving statistical analysis will take place following iGEM Americas Jamboree.	+	[[Image:Ox1rptfinal.png|thumb|569px|center]]
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	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

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Latest revision as of 13:33, 6 November 2011

ODR7:OX1R:UNC-54 (for use in C. elegans)

Composite part designed to modify chemotaxis of C. elegans.

Characterization
Part was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. Target chemical used included naphthalene, pond tailings sample, bitumen sample, and 4-chlorobenzoic acid.

Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. Any worm contained in the circular zone approximately 0.5mm around centre of agar plate was not counted as either in a control or target quadrant. An example agar plate is shown below.

To determine quantitative measurement, the chemotactic index was determined. The formula for this index is shown below:

Result

Sequence and Features