Difference between revisions of "Part:BBa K592032:Experience"

Latest revision as of 17:12, 5 November 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K592032

User Reviews

•

iGEM2011_Uppsala-Sweden

This part used BBa_E0034 EYFP. However, in our experiments we used the newer BBa_K592101 YFP which had been codon optimized for expression in yeast. It also works well in E.coli. As a result, parts with BBa_K592101 show higher fluorescent activity when measured, probably due to better expression.

UNIQ385ee40c8f979fbb-partinfo-00000003-QINU

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 <I>iGEM2011_Uppsala-Sweden</I>
 |width='60%' valign='top'|
-This part used <partinfo>BBa_E0034</partinfo> EYFP which showed much weaker fluorescent activity than e.g. the newer <partinfo>BBa_K592101</partinfo> YFP. As a result, this part and its design is not very good for testing promoter strength. Recommend using stronger fluorescent proteins as reporters.
+This part used <partinfo>BBa_E0034</partinfo> EYFP. However, in our experiments we used the newer <partinfo>BBa_K592101</partinfo> YFP which had been codon optimized for expression in yeast. It also works well in E.coli. As a result, parts with BBa_K592101 show higher fluorescent activity when measured, probably due to better expression.
 |}
 <!-- DON'T DELETE --><partinfo>BBa_K592032 EndReviews</partinfo>