Difference between revisions of "Part:BBa K511813"

 
 
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<partinfo>BBa_K511813 short</partinfo>
 
<partinfo>BBa_K511813 short</partinfo>
  
 
This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-4xMnt promoter. In the absence of Mnt-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderate.
 
This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-4xMnt promoter. In the absence of Mnt-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderate.
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https://static.igem.org/mediawiki/parts/d/de/Mntvp16.jpg
  
 
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Latest revision as of 16:07, 5 November 2011

Mnt-VP16-Inducible Red Fluorescence Generator (minCMV-4xMnt-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate driven by the minCMV-4xMnt promoter. In the absence of Mnt-VP16, transcription of mKate is very low, but in the presence of CI434-VP16, transcription is moderate.

Mntvp16.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal BglII site found at 110
    Illegal BglII site found at 181
    Illegal BglII site found at 252
    Illegal XhoI site found at 1
    Illegal XhoI site found at 72
    Illegal XhoI site found at 143
    Illegal XhoI site found at 214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1003
    Illegal SapI.rc site found at 385