Difference between revisions of "Part:BBa K511900"

 
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K511900 short</partinfo>
 
<partinfo>BBa_K511900 short</partinfo>
  
 
This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level.
 
This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level.
 +
 +
Graph showing the activation of UAS-Gal4_EYFP-4xFF4 by a constitutively expressed Gal4-VP16
 +
 +
https://static.igem.org/mediawiki/parts/2/2e/Uas_eyfp-4xff4_hef1a_gv16.jpg
  
  

Latest revision as of 15:56, 5 November 2011

Gal4-VP16 Transactivator Generator (Hef1a-Gal4-VP16) MammoBlock Device

This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level.

Graph showing the activation of UAS-Gal4_EYFP-4xFF4 by a constitutively expressed Gal4-VP16

Uas_eyfp-4xff4_hef1a_gv16.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
    Illegal XhoI site found at 1726
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1645