Difference between revisions of "Part:BBa K511900"
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<partinfo>BBa_K511900 short</partinfo> | <partinfo>BBa_K511900 short</partinfo> | ||
This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level. | This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level. | ||
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+ | Graph showing the activation of UAS-Gal4_EYFP-4xFF4 by a constitutively expressed Gal4-VP16 | ||
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+ | https://static.igem.org/mediawiki/parts/2/2e/Uas_eyfp-4xff4_hef1a_gv16.jpg | ||
Latest revision as of 15:56, 5 November 2011
Gal4-VP16 Transactivator Generator (Hef1a-Gal4-VP16) MammoBlock Device
This MammoBlock composite device produces the Gal4-VP16 transactivator at a low, constitutive level.
Graph showing the activation of UAS-Gal4_EYFP-4xFF4 by a constitutively expressed Gal4-VP16
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014
Illegal XhoI site found at 1726 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1645