Difference between revisions of "Part:BBa K602016"
 
Line 3: Line 3:
  
 
<i>D. radiodurans</i> PprI and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
 
<i>D. radiodurans</i> PprI and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
−
 
  
 
'''''Please see Experience page for characterization details.'''''
 
'''''Please see Experience page for characterization details.'''''
 +
  
 
'''''NOTE: There is a double terminator (<partinfo>BBa_B0015</partinfo>) between the PprI CDS (K602000) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.'''''
 
'''''NOTE: There is a double terminator (<partinfo>BBa_B0015</partinfo>) between the PprI CDS (K602000) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.'''''

Latest revision as of 01:33, 5 November 2011

D. radiodurans PprI+RecA expression device

D. radiodurans PprI and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

Please see Experience page for characterization details.


NOTE: There is a double terminator (BBa_B0015) between the PprI CDS (K602000) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2348
  • 1000
    COMPATIBLE WITH RFC[1000]