Difference between revisions of "Part:BBa K602020"
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<i>D. radiodurans</i> PprM and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
 
<i>D. radiodurans</i> PprM and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
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'''''Please see Experience page for characterization details.'''''
  
 
'''''NOTE: There is a double terminator (<partinfo>BBa_B0015</partinfo>) between the PprM CDS (K602002) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.'''''
 
'''''NOTE: There is a double terminator (<partinfo>BBa_B0015</partinfo>) between the PprM CDS (K602002) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.'''''

Revision as of 01:33, 5 November 2011

D. radiodurans PprM+RecA expression device

D. radiodurans PprM and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

Please see Experience page for characterization details.

NOTE: There is a double terminator (BBa_B0015) between the PprM CDS (K602002) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1089
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1763
  • 1000
    COMPATIBLE WITH RFC[1000]