Difference between revisions of "Part:BBa K568008"

Revision as of 10:39, 2 November 2011

Red light sensor with GFP reporter

Red light sensor with GFP reporter. For more information and references check the red light sensor motherpart (BBa_K568000). However, the data obtained did not match our expectations. We tested its function in JT2. The test of the assay was repeated several times. Our results indicate that the part is independed of the different light conditions active.

Therefore, testing or even the design of this part should be revised.

Experimental testing

The sensor was tested after incubation overnight under the on-light of 705 nm, off-light of 650 nm, in ambient light and in the dark.

Another assay was set up to test the time dependency of the construct which did not alter our findings. In this experiment, the cells had been incubated over night in the dark and had been transferred into the different lighting conditions at t = 0 min. The construct yielded more GFP output than the constitutive promoter at all times.

Sequence and Features