Difference between revisions of "Part:BBa K553010:Experience"

(Applications of BBa_K553010)
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===Applications of BBa_K553010===
 
===Applications of BBa_K553010===
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UNITS_Trieste Synbiome project
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bacteria Intra-strain communication:
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An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule. The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria.
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The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.
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[[Image:Coculture_assay.jpg‎]]
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The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 10:16, 1 November 2011

Datasheet download - experience inside: Media:K553010_lasIcost.pdf‎

Applications of BBa_K553010

UNITS_Trieste Synbiome project

bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule. The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria.

The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.

Coculture assay.jpg

The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.

User Reviews

UNIQc95a364d574ce4de-partinfo-00000000-QINU UNIQc95a364d574ce4de-partinfo-00000001-QINU