Difference between revisions of "Part:BBa K590015"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 10: | Line 10: | ||
We transformed this part into an ''E. coli'' BL-21 lacI<sup>q</sup> strain and observed fluorescent filament structures that in some cases, prevented cells from dividing properly. The same construct has been successfully cloned into Gibson assembly vectors pGA1A3 and pGA4C5. | We transformed this part into an ''E. coli'' BL-21 lacI<sup>q</sup> strain and observed fluorescent filament structures that in some cases, prevented cells from dividing properly. The same construct has been successfully cloned into Gibson assembly vectors pGA1A3 and pGA4C5. | ||
| − | [[Image:MamK sfGFP 3k3-1 uwigem2011part.jpg| | + | [[Image:MamK sfGFP 3k3-1 uwigem2011part.jpg|300px|center]] |
<html><center> | <html><center> | ||
<iframe width="420" height="315" src="http://www.youtube.com/embed/BLLyUHrrcV4" frameborder="0" allowfullscreen></iframe> | <iframe width="420" height="315" src="http://www.youtube.com/embed/BLLyUHrrcV4" frameborder="0" allowfullscreen></iframe> | ||
Latest revision as of 18:20, 31 October 2011
sfGFP_mamK_pGA1C3
This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].
Usage and Biology
This part consists of the mamK gene from Magnetospirillum magneticum strain AMB-1, fused to superfolder gfp on pGA1C3. MamK has been reported to be essential for proper magnetosome formation in magnetotactic bacteria; its bacterial actin-like cytoskeleton protein is required for proper alignment of the magnetosomes in a chain. Beyond magnetosome alignment, MamK filaments could act as a scaffold for protein localization or to alter cell morphology by preventing cells from fully dividing (see images below).
We transformed this part into an E. coli BL-21 lacIq strain and observed fluorescent filament structures that in some cases, prevented cells from dividing properly. The same construct has been successfully cloned into Gibson assembly vectors pGA1A3 and pGA4C5.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1430
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1430
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1134
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1430
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1430
Illegal NgoMIV site found at 1156
Illegal NgoMIV site found at 1523
Illegal NgoMIV site found at 1670 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 80
Illegal SapI.rc site found at 1802