Difference between revisions of "Part:BBa K510044"
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For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.
 
For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.
  
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[[Image:UPOSevilla-miniTn7-basic_flipflop-pellets.jpg|400px|center]]
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Figure. '''Fluorescent pellets containing the basic flip-flop device'''. Pellets of E. coli strains containing the basic flip-flop in the miniTn7BB-Gm transposon in medium copy number (R6K-based vector -part BBa_K510044-, left), high copy number (pUC18-based vector -part BBa_K510043-, center), or not containing the flip-flop device (right). It is quite interesting that the basal state of the basic flip-flop is red in the medium copy number plasmid and green in the high copy number plasmid.
  
 
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Revision as of 19:08, 29 October 2011

pUC18R6KT-miniTn7BB-Gm-bistable_switch


This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm (BBa_K510012)characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.

For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.

UPOSevilla-miniTn7-basic flipflop-pellets.jpg

Figure. Fluorescent pellets containing the basic flip-flop device. Pellets of E. coli strains containing the basic flip-flop in the miniTn7BB-Gm transposon in medium copy number (R6K-based vector -part BBa_K510044-, left), high copy number (pUC18-based vector -part BBa_K510043-, center), or not containing the flip-flop device (right). It is quite interesting that the basal state of the basic flip-flop is red in the medium copy number plasmid and green in the high copy number plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BglII site found at 7615
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 8202
    Illegal AgeI site found at 8314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal BsaI.rc site found at 6171
    Illegal SapI site found at 518