Difference between revisions of "Part:BBa K567027"
 
Line 2: Line 2:
 
<partinfo>BBa_K567027 short</partinfo>
 
<partinfo>BBa_K567027 short</partinfo>
  
 +
This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 4 AGG codons and 2 GCG codons are inserted in THREE DIFFERENT places after the ATG start codon of wild type luciferase (BBa_I712019) with an interval of 30bp and 111bp respectively.
  
 
=='''Related Biobrick'''==
 
=='''Related Biobrick'''==

Latest revision as of 03:56, 29 October 2011

PT7-Luc-3x4AGG(30bp+111bp)

This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 4 AGG codons and 2 GCG codons are inserted in THREE DIFFERENT places after the ATG start codon of wild type luciferase (BBa_I712019) with an interval of 30bp and 111bp respectively.

Related Biobrick

lacI-Ptrc-tRNAArg (BBa_K567001)

PT7-Luc-2x4AGG(30bp) (BBa_K567026)

PT7-Luc-3x4AGG(30bp+111bp) (BBa_K567027)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1091