Difference between revisions of "Part:BBa K572100"
 
Line 16: Line 16:
 
<!-- -->
 
<!-- -->
 
<html>
 
<html>
−
<img src="https://static.igem.org/mediawiki/parts/3/3e/PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX">
+
<img src="https://static.igem.org/mediawiki/parts/d/de/PSB1Pc.png" WIDTH="843PX" HEIGHT="648PX">
 
</HTML>
 
</HTML>

Latest revision as of 01:51, 29 October 2011

Light based screening plasmid backbone - pSB1Pc

The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2015
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2139
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2139
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2154
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.