Difference between revisions of "Part:BBa K567022"
Line 2: Line 2:
 
<partinfo>BBa_K567022 short</partinfo>
 
<partinfo>BBa_K567022 short</partinfo>
  
−
This part is constructed based on PT7-Luc-2AGG [https://parts.igem.org/wiki/index.php?title=Part:BBa_K567008 BBa_K567008]
+
This part is constructed based on PT7-Luc-2AGG ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567008 BBa_K567008])
 +
The transcription of this part is under the control of T7 promoter and ''lac'' operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:50, 29 October 2011

PT7-Luc-2AGG-tRNA(Arg)

This part is constructed based on PT7-Luc-2AGG (BBa_K567008) The transcription of this part is under the control of T7 promoter and lac operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1923
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 911