Difference between revisions of "Part:BBa K648125"

 
 
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<partinfo>BBa_K648125 short</partinfo>
 
<partinfo>BBa_K648125 short</partinfo>
  
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This is a test circuit comprised of GFP, mCherry, operating region (Or) of lambda phage, pBAD, and the cI repressor with RBS B0030.  When the circuit is induced, the cI repressor is expressed.  cI repressor will bind to the binding sites of the operating region.   
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This is a test circuit comprised of GFP, mCherry, operating region (Or) of lambda phage, pBAD, and the cI repressor with RBS B0030.  When the circuit is induced, the cI repressor is expressed.  cI repressor will bind to the binding sites of the operating region.  The GFP used is the reverse sequence.   
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:26, 29 October 2011

Or tester with cI repressor with slightly weak RBS

This is a test circuit comprised of GFP, mCherry, operating region (Or) of lambda phage, pBAD, and the cI repressor with RBS B0030. When the circuit is induced, the cI repressor is expressed. cI repressor will bind to the binding sites of the operating region. The GFP used is the reverse sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644