Difference between revisions of "Part:BBa K537009"
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<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/2/27/Datasheet1.pdf">here</a></big></p> | <p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/2/27/Datasheet1.pdf">here</a></big></p> | ||
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Revision as of 19:26, 28 October 2011
Promoter-Theophylline riboswitch 1-Venus-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 729
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
For the pdf version of this datasheet, click here
