Difference between revisions of "Part:BBa K656010"
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This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not. | This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not. | ||
| − | [[Image:File- | + | We transformed Anabaena 7120 with a construct containing GFP reporter controlled by psaC promoter part BBa_K656010. Bright field light micrograph and GFP filter images are compared to demonstrate fluorescence of a successful transformant. Red arrows point to heterocyst cells containing noticeably less fluorescence. |
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| + | [[Image:File-Ana trans bright field.JPG|600px|center|thumb|Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010, Bright field image]] | ||
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| + | [[Image:File-Ana trans GFP marked heterocysts.JPG|600px|center|thumb|Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010, GFP filter; Red arrows point to heterocysts]] | ||
Revision as of 17:29, 28 October 2011
Anabaena pSac Promoter
Anabaena vegetative cell promoter, linked to photosystem I. Can be used to express specifically in vegetative cells, excluding heterocyst expression.
This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.
We transformed Anabaena 7120 with a construct containing GFP reporter controlled by psaC promoter part BBa_K656010. Bright field light micrograph and GFP filter images are compared to demonstrate fluorescence of a successful transformant. Red arrows point to heterocyst cells containing noticeably less fluorescence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]