Difference between revisions of "Part:BBa K656010"

Revision as of 17:25, 28 October 2011

Anabaena pSac Promoter

Anabaena vegetative cell promoter, linked to photosystem I. Can be used to express specifically in vegetative cells, excluding heterocyst expression.

This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.

File:File-Marked heterocysts.JPG

Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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	This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.		This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.
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		+	[[Image:File-Marked heterocysts.JPG\|600px\|center\|thumb\|Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010]]