Difference between revisions of "Part:BBa K649101"

Revision as of 14:33, 28 October 2011

PlsrR-lsrR

LsrR is the repressor of the lsr operon and itself. Auto Inducer 2(AI-2) is phosphorylated by LsrK, and phospho AI-2 relieves LsrR repression.

This part contains lsrR and lsrK gene, downstream of lsrR promoter. lsrR promoter and lsrR coding gene in this part work correctly, but lsrK has flame shift mutation and does not work properly.

To confirm LsrR represses lsrA promoter, we constructed BBa_K649105.

Fluorescence intensity is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed promoter lsrA. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.

For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1725
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1399
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 To confirm LsrR represses lsrA promoter, we constructed BBa_K649105.
-[[Image:LsrR_repression2.png|thumb|center|500px|Fluorescence intensity is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
+[[Image:LsrR_repression3.png|thumb|center|500px|Fluorescence intensity is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
 We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a <i>lsrR</i> gene downstream of lsrA promoter-<i>gfp</i>.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed promoter lsrA. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.