Difference between revisions of "Part:BBa K537003"
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This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region. | This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K537003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K537003 SequenceAndFeatures</partinfo> | ||
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+ | <p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/2/27/Datasheet1.pdf">here</a></big></p> | ||
+ | <center><img src="https://static.igem.org/mediawiki/igem.org/5/5b/Datasheet1.jpg" width="910" vspace="15"></center> | ||
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+ | </html> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K537003 parameters</partinfo> | <partinfo>BBa_K537003 parameters</partinfo> | ||
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Revision as of 12:58, 28 October 2011
Theophylline Riboswitch 1-Venus
This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptamer specific to theophylline.
This theophylline riboswitch (type1) biobrick activates the expression of the Venus fluorescent protein in a theophylline-dependent fashion. It is composed of a theophylline sensitive riboswitch clone 8.1 (Topp and Gallivan JACS 2007; BBa_K249026 and BBa_K411001) fused upstream to an adjacent Venus coding region.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 686
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
For the pdf version of this datasheet, click here