Difference between revisions of "Part:BBa K649100"
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<partinfo>BBa_K649100 short</partinfo>
 
<partinfo>BBa_K649100 short</partinfo>
  
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BBa_K649100 contains lsrA promoter and RBS. We did not identified where RBS is in this part. But BBa_K649104, composed of this part and ''gfp'' gene, worked properly. So this part has a region works as RBS.</br></br>
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BBa_K649100 contains lsrA promoter and RBS. We did not identified where RBS is in this part. But BBa_K649104, composed of this part and ''gfp'' gene, worked properly. So this part has a region works as RBS.<br>
 
lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.
 
lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.
  

Revision as of 12:57, 28 October 2011

LsrA promoter

BBa_K649100 contains lsrA promoter and RBS. We did not identified where RBS is in this part. But BBa_K649104, composed of this part and gfp gene, worked properly. So this part has a region works as RBS.
lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.


To characterize this promoter, we constructed the reporter part BBa_K649104. If you want to see detailed infomation, please click here.


We inproved previous lsrA promoter(BBa_K117002)).our assay of BBa_K117002


For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]