Difference between revisions of "Part:BBa K537009"
Line 3: Line 3:
  
 
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
 
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
−
<html>
+
 
−
<center><img src="https://static.igem.org/mediawiki/2011/7/73/Datasheet1.JPG" width="900" vspace="15"></center>
+
−
<p>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/2011/2/27/Datasheet1.pdf">here</a></p>
+
−
</html>
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
Line 14: Line 11:
 
<partinfo>BBa_K537009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K537009 SequenceAndFeatures</partinfo>
  
 +
<html>
 +
</br>
 +
</br>
 +
<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/2/27/Datasheet1.pdf">here</a></big></p>
 +
<center><img src="https://static.igem.org/mediawiki/igem.org/5/5b/Datasheet1.jpg" width="910" vspace="15"></center>
  
 +
 +
</html>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K537009 parameters</partinfo>
 
<partinfo>BBa_K537009 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 12:56, 28 October 2011

Promoter-Theophylline riboswitch 1-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



For the pdf version of this datasheet, click here