Difference between revisions of "Part:BBa K523024"

 
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__NOTOC__
 
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<partinfo>BBa_K523024 short</partinfo>
 
<partinfo>BBa_K523024 short</partinfo>
  
Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species.
+
Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species. The part contains, in order:
 +
 
 +
* The GH10 exoglucanase domain
 +
* A (possibly inactivated) cellulose binding domain
 +
* The GH6 endoglucanase domain
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:13, 28 October 2011

cex + cenA fusion (cxnA)

Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The part contains, in order:

  • The GH10 exoglucanase domain
  • A (possibly inactivated) cellulose binding domain
  • The GH6 endoglucanase domain

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 524
    Illegal NotI site found at 2222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2135
    Illegal XhoI site found at 1633
    Illegal XhoI site found at 1882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 530
    Illegal NgoMIV site found at 1032
    Illegal NgoMIV site found at 1413
    Illegal NgoMIV site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI.rc site found at 660