Difference between revisions of "Part:BBa K602011"

Latest revision as of 10:18, 28 October 2011

D. radiodurans radioresistance genes expression device

D. radiodurans PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

Note that as there is no terminator between each CDS, each transcript will contain all four genes as well as their respective ribosome binding sites. Assuming equal binding to each RBS, genes that come later in the sequence are expected to be translated more frequently (RecA > PprM > PprA > PprI) due to ribosome read-through.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2829
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3503
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K602011 short</partinfo>
 <i>D. radiodurans</i> PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.
+Note that as there is no terminator between each CDS, each transcript will contain all four genes as well as their respective ribosome binding sites. Assuming equal binding to each RBS, genes that come later in the sequence are expected to be translated more frequently (RecA > PprM > PprA > PprI) due to ribosome read-through.
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