Difference between revisions of "Part:BBa K602017"

 
 
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<partinfo>BBa_K602017 short</partinfo>
 
<partinfo>BBa_K602017 short</partinfo>
  
 
<i>D. radiodurans</i> PprA and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
 
<i>D. radiodurans</i> PprA and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.  
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'''''NOTE: There is a double terminator (<partinfo>BBa_B0015</partinfo>) between the PprA CDS (K602001) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.'''''
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 09:58, 28 October 2011

D. radiodurans PprA+RecA expression device

D. radiodurans PprA and RecA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

NOTE: There is a double terminator (BBa_B0015) between the PprA CDS (K602001) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1590
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2264
  • 1000
    COMPATIBLE WITH RFC[1000]