Difference between revisions of "Part:BBa K560016"

Revision as of 13:08, 27 October 2011

BsaI Golden Gate Assembly_Backbone

The backbone for golden gate assembly. This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012[1], BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal. More details about experiment protocol can be found in our wiki. Details about the structure of this plasmid can be found in http://www.addgene.com/31021/.

The Genetic Map

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 35
Illegal NheI site found at 2776
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 363
Illegal XhoI site found at 1797
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644
Illegal NgoMIV site found at 1650
Illegal AgeI site found at 569
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 575
Illegal BsaI.rc site found at 128
Illegal SapI.rc site found at 2653

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 The backbone for golden gate assembly.
-This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K560012">BBa_K560012</a>, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal.
+This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012[https://parts.igem.org/wiki/index.php?title=Part:BBa_K560012], BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal.
 More details about experiment protocol can be found in our wiki.
 Details about the structure of this plasmid can be found in http://www.addgene.com/31021/.