Difference between revisions of "Part:BBa K606040"

Latest revision as of 08:45, 26 October 2011

Promoter Hyperspank B.subtilis & E.coli

Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).

This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part K143055.

Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed K606010. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis. Otherwise, the promoter is going to act as a constitutive one.

Please note that this promoter also works fine in E. coli, and can be use to build more universal parts, compatible with both B. Subtilis and E. Coli devices, since the promoter have approximtely the same behaviour in both.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part [https://parts.igem.org/Part:BBa_K143055 K143055].
-Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed [https://parts.igem.org/Part:BBa_K606010 K606010]. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis.
+Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed [https://parts.igem.org/Part:BBa_K606010 K606010]. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis. Otherwise, the promoter is going to act as a constitutive one.
-Please note that this promoter also works fine in E. colI.
+Please note that this promoter also works fine in E. coli, and can be use to build more universal parts, compatible with both B. Subtilis and E. Coli devices, since the promoter have approximtely the same behaviour in both.
 <!-- Add more about the biology of this part here