Difference between revisions of "Part:BBa K606040"
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Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). | Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). | ||
| − | This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part [https://parts.igem.org/Part:BBa_K143055 K143055] | + | This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part [https://parts.igem.org/Part:BBa_K143055 K143055]. |
Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed [https://parts.igem.org/Part:BBa_K606010 K606010]. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis. | Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed [https://parts.igem.org/Part:BBa_K606010 K606010]. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis. | ||
Revision as of 22:19, 25 October 2011
Promoter Hyperspank B.subtilis & E.coli
Promoter hyper-spank is an IPTG inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG).
This promoter is flanked by two LacO sites. One downstream to make road block, and one upstream to prevent the binding of the sigma subunit. This part is similar but not exactly the same than the part K143055.
Note: Since there is not a sufficient expression of the LacI gene in subtilis, we strongly recomand you to add a LacI expression cassette for subtilis, such as the one we designed K606010. This is a strong expression casset, with which you expect a low response with IPTG concentration, used for a better control of the level of expression of the genes in B. subtilis.
Please note that this promoter also works fine in E. colI.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]