Difference between revisions of "Part:BBa K606056"
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<partinfo>BBa_K606056 short</partinfo> | <partinfo>BBa_K606056 short</partinfo> | ||
| − | Lambda phage attR recombination site cloned with a double terminator. | + | Lambda phage attR recombination site from the lambda phage cloned with a double terminator on the left. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3. |
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| + | The idea is that this part can be used in combination with the part [https://parts.igem.org/Part:BBa_K606056 K606049]. When expressing the Xis protein, the stop can be excised, and the transcription recovered. | ||
| + | [[Image:Excision_xis-reporter.jpg|thumb|center]] | ||
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| + | The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature. | ||
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Latest revision as of 21:54, 25 October 2011
Double terminator + Lambda phage attR recombination site
Lambda phage attR recombination site from the lambda phage cloned with a double terminator on the left. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3.
The idea is that this part can be used in combination with the part K606049. When expressing the Xis protein, the stop can be excised, and the transcription recovered.
The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]