Difference between revisions of "Part:BBa K606061"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K606061 short</partinfo> | <partinfo>BBa_K606061 short</partinfo> | ||
− | SpoVG Ribosome Binding Site (RBS) for B. subtilis | + | SpoVG Ribosome Binding Site (RBS) for B. subtilis. |
+ | |||
+ | This is a strong ribosome binding site from suibtilis. This part is a copy of the part from Imperial college 2008, BBa_K143021, but we have synthetized it de-novo, using primer matching technique, and submitted it to the registry. | ||
+ | |||
+ | This parts also works fine in E. coli. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | This part can be use to express proteins in both B. subtilis and E. coli, and leads to a strong expression of the protein. The original sequence come from the reference following reference [1] | ||
+ | |||
+ | ===References=== | ||
+ | <biblio> | ||
+ | #1 pmid=9669336 | ||
+ | </biblio> | ||
+ | |||
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Revision as of 20:50, 25 October 2011
SpoVG Ribosome Binding Site (RBS) for B. subtilis
SpoVG Ribosome Binding Site (RBS) for B. subtilis.
This is a strong ribosome binding site from suibtilis. This part is a copy of the part from Imperial college 2008, BBa_K143021, but we have synthetized it de-novo, using primer matching technique, and submitted it to the registry.
This parts also works fine in E. coli.
Usage and Biology
This part can be use to express proteins in both B. subtilis and E. coli, and leads to a strong expression of the protein. The original sequence come from the reference following reference [1]
References
<biblio>
- 1 pmid=9669336
</biblio>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]