Difference between revisions of "Part:BBa K642007:Design"

(→‎Source)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal1 promoter. The Gal10/1 promoter is commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.
+
The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal1 promoter. The Gal1 promoter is commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.
  
 
===References===
 
===References===

Revision as of 21:01, 24 October 2011

Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 89
    Illegal BsaI.rc site found at 1379


Design Notes

The yeGFP part (BBa_K319039) from last year didn't have a stop codon. In PCR amplification of this part, we added a stop codon.

Source

The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005 and using overlap extension PCR was fused to the amplified proximal region of the Gal1 promoter. The Gal1 promoter is commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.

References